In the vCenter user interface, in the Hosts and Clusters view, with the ESXi host selected, you see the message "vSphere HA agent on this host could not reach isolation address <IP Address>
On a regular basis HA contacts ( using ICMP ping ) its isolation response address to ensure the cluster is functioning correctly. The default isolation response address is the default gateway but other reliable IP addresses can be configured along with the default gateway or the default gateway can also be disabled as an isolation response address.
This issue can occur when HA does not receive a response from all of its configured isolation response addresses.
To resolve this issue, investigate your network to determine why HA is unable to receive a response from at least one isolation response address.
Note:
For more information on using CLI command: vmkping, please reference the following article: Troubleshooting network and TCP/UDP port connectivity issues on Hosts
After confirming network connectivity is possible to the Isolation address from the hosts vmk interface and the error message is still presenting on the ESXi host, please proceed with verifying: vSphere HA agent on this host could not reach isolation address '#.#.#.#', reported on ESXi host