Select VMs are showing higher then expected latency on the VM level and in the guest OS
The majority of other VMs are not showing the same latency and there is no evidence of vSAN storage latency.
vSAN (All)
VM workload is presenting very large I/O sizes up to 512k or larger
A review of this can be done using vSAN I/O Insight and this will show a large amount of I/O that is not 4k aligned and/or the larger I/O sizes generating the larger latency.
To use vSAN I/O Insight
The IO size should be small (ideally 4K) for minimum latency and maximum IOPs. On the other hand, the IO size should be large (ideally 256K) for the goal of maximum throughput.
Please adjust the IO size according to the desired goal of performance evaluation. Keep in mind that vSAN is optimized for 64K and smaller I/O sizes; configuring an I/O size >64K will result in increased guest I/O latency.
For additional guidance on I/O insight see the following:
Use vSAN I/O Insight
View vSAN I/O Insight Metrics
This is also related to the following KB and diagnostics report:
vSAN performance diagnostics reports: "The size of IOs may not be optimal to achieve the desired goal"