VMware NSX
VMware HCX
Packet captures on the impacted VM's Guest OS confirms, the TCP SYN packet being received by the source VM but no TCP SYN,ACK response is being responded by impacted VM (Virtual Machine).
The issue appears to be outside the scope of ESXi or the virtual machine itself. We recommend contacting the Guest OS vendor for further assistance.
Workaround: A new VM was created with the same Guest OS to use same vLAN, and RDP functionality worked as expected.