vSphere HA agent on this host could not reach isolation address <IP Address>"The ESXi host is unable to establish network connectivity to the defined isolation IP address indicated in the error message.
vmkping -I vmk<#> <Isolation_Address>
For more information on using CLI to test the connectivity Refer: Troubleshooting network and TCP/UDP port connectivity issues on Hosts
After confirming network connectivity is possible to the Isolation address from the hosts' vmk interface and the error message persists on the ESXi host, then refer: vSphere HA agent on this host could not reach isolation address '#.#.#.#', reported on ESXi host