VMware NSX
This is due to the VM in question not being powered on and not connected to an NSX overlay segment.
Ensure the VM being selected in Traceflow is powered on and connected to an NSX overlay segment.
Troubleshooting NSX Traceflow
For enabling Traceflow on VLAN segments: Traceflow attempt in NSX GUI fails with " Traceflow on VLAN logical port InternalLogicalPort/<portID> requires INT (In-band Network Telemetry) to be enabled."