When PCI vSphere DirectPath I/O devices are made available to a virtual machine, you cannot perform certain operations on the virtual machine
You cannot live migrate this VM to another host.
No snapshot operations are allowed.
The VM cannot be suspended or resumed.
VMware vSphere vCenter
-When PCI vSphere DirectPath I/O devices are made available to a virtual machine, you cannot perform certain operations on the virtual machine. These operations include suspending, migration with vMotion, and taking or restoring snapshots of the virtual machine.
Remove PCIe Passthrough (DirectPath I/O)
If PCIe passthrough is no longer required, follow the steps below to safely remove the device.
Shutdown the VM
vMotion operation will work post removing PCIe Passthrough (DirectPath I/O) device from the Virtual Machine
Reference techdocs link for PCI Device: