VMware vSAN (All Versions)
Due to failed certificate updates from self signed to custom certificates on all hosts in the cluster, this results in vCenter not trusting/verifying the host certificates for the hosts, which resulted in 2 of the hosts getting removed from the vSAN unicast agent list. The hosts were removed due to vCenter being authoritative for pushing vSAN updates to ESXi.
Confirm vCenter is still authoritative from all hosts in the vSAN cluster by running the following:
esxcfg-advcfg -g /VSAN/IgnoreClusterMemberListUpdates
Value of 0 means vCenter is authoritative.
Follow KB Configuring vSAN Unicast networking from the command line, to manually add the missing host's unicast agent list entries to each of the affected hosts.