When the ISO is mounted in a virtual machine running on an ESXi/ESX host, a file lock is maintained on the ISO file. Each host connection to the file consumes one lock. There is a maximum of eight locks possible for a file residing on a VMFS 3 datastore. This limits the amount of virtual machine hosts that can access a disk to a cluster of eight ESX/ESXi hosts. This restriction applies to virtual disk (VMDK) files, ISO CD/DVD images, floppy images, or any other file.
Note: The number of virtual machines running on the hosts and accessing the same virtual disk can exceed eight.