This issue occurs when the backup virtual machine and the VCB-HELPER virtual machine are residing on datastores of different block sizes. This issue occurs when the .vmdk files of the backup virtual machine are greater than the maximum file size supported on the datastore of VCB-HELPER virtual machines. To work around this issue, ensure VCB-HELPER virtual machines and the backup virtual machine reside on datastores of the same block size.
When choosing a datastore on which to store the files for the backup appliance, choose the datastore with the largest VMFS block size. This is necessary to ensure that the backup appliance can back up virtual machines from all datastores.