This article provides configuration information when using an EtherChannel (or port channel) for connectivity between ESXi hosts and Physical Network Switches on a vSphere Standard Switch (vSS) or vSphere Distributed Switch (vDS).
To configure the ESXi portion of a port channel, the aggregated NICs need to be placed into a load balancing configuration of "Route based on IP hash". This can be done at a virtual switch level or at an individual portgroup level, however the only use case for modifying only certain portgroups would be if both standalone and port channeled NICs are on the same vSwitch, which is an unsupported configuration: Route Based on IP Hash
Note: If the physical network is configured with an EtherChannel (port channel) do not create a LAG within the virtual Distributed Switch. A LAG is only needed when the physical network is using LACP. Please see Configuring LACP on a vSphere Distributed Switch Port Group
For more information about EtherChannel (port channel) configurations and other link aggregation concepts please reference the articles below: