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C:\Program Files\Altiris\Notification Server\web.config
Ensure the following accounts have "Read & Execute" and "Read" permissions:
If permissions to this file have been modified to not include any of these accounts, then it is likely you will need to review further permissions. You may refer to Knowledgebase Article #17551 for further permission assignment details.
"Users" or "Authenticated Users" or "NETWORK SERVICE" or "ASPNET" and "IIS_WPG"